Marinkovich Laboratory IN THE DEPARTMENT OF DERMATOLOGY

Engineering laminin molecules

scanning EM


Structure-function analysis of extracellular matrix molecules from simple processes such as cell adhesion to complex processes such as carcinoma invasion, is a major focus of the Marinkovich lab. This figure shows keratinocytes engineered to express wild type laminin-332 (WT), laminin-332 containing deletions of domain VI (ΔVI) or domains Vi-III , compared to keratinocytes with absent laminin-332 expression (Null). Scanning electron microscopy and transmission electron microscopy focused on the interface of cell and substratum shows increasing areas of discontinuity (arrows) corresponding to decreased adhesion.

 

References:

Sakai, N., E.A. Waterman, N.T. Nguyen, D.R. Keene and M. P. Marinkovich (2010). "Observations of Skin Grafts Derived from Keratinocytes Expressing Selectively Engineered Mutant Laminin-332 Molecules." J Invest Dermatol.

Ko, M. S. and M. P. Marinkovich (2010). "Role of dermal-epidermal basement membrane zone in skin, cancer, and developmental disorders." Dermatol Clin 28(1): 1-16.

 

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